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Brain Res Bull. 2008 Mar 18;75(2-4):379-83. doi: 10.1016/j.brainresbull.2007.10.026. Epub 2007 Nov 20.

Calbindin-D28k and calretinin as markers of retinal neurons in the anuran amphibian Rana perezi.

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Department of Cell Biology, Faculty of Biology, University Complutense, 28040 Madrid, Spain.


In the present study we have analyzed the distribution of the calcium binding proteins calbindin-D28k (CB) and calretinin (CR) immunoreactive cells in the retina of the anuran Rana perezi using poly- and mono-clonal antibodies that were proven to be specific in the amphibian brain, without cross-reactivity. Double immunohistofluorescence techniques were used to demonstrate colocalization of both proteins in the same retinal cells. In addition, retrograde tracing experiments from the optic nerve were conducted to labeled ganglion cells and these were observed in combination with CB and/or CR immunohistochemistry. Cells containing CB were identified as all cones, scattered bipolar and amacrine cells together with cells in the ganglion cell layer. The pattern of CR immunoreactivity was strikingly different. Abundant cells contained CR in the inner retinal layers including horizontal, bipolar and amacrine cells, and cells in the ganglion cell layer. By means of double immunohistochemistry it was found that only subpopulations of amacrine cells and cells in the ganglion cell layer contained both CB and CR. Tracing from the optic nerve revealed retrogradely labeled ganglion cells with different morphologies and most of them contained CB and/or CR. All these data taken together suggest that in amphibians CB and CR are distinctly and precisely distributed in retinal neurons showing, however, peculiar features not observed previously in other vertebrates.

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