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Arch Biochem Biophys. 2008 May 1;473(1):88-95. doi: 10.1016/j.abb.2008.02.027. Epub 2008 Mar 4.

Redox and heavy metal effects on the biochemical activities of an Arabidopsis polyadenylation factor subunit.

Author information

1
Department of Plant and Soil Sciences, University of Kentucky, 301A Plant Science Building, 1405 Veterans Drive, Lexington, KY 40546-0312, USA.

Abstract

The Arabidopsis CPSF30 ortholog (AtCPSF30) is an RNA-binding endonuclease that is part of the plant polyadenylation complex. Previous work (B. Addepalli, A.G. Hunt, Nucleic Acids Res. 35 (2007) 4453-4463) demonstrated that different zinc finger motifs in the protein were responsible for RNA-binding and nuclease activity, respectively. In this study, a more detailed functional map of AtCPSF30 is presented, a map that includes descriptions of novel biochemical activities. Elevated temperatures, the specific zinc chelator 1,10-phenanthroline, and the sulfhydryl reagent dithiothreitol all had differential inhibitory effects on the RNA-binding and nuclease activities. The endonuclease activity of AtCPSF30 was inhibited by relatively high (>100muM) concentrations of zinc, and this inhibition required a plant-specific N-terminal domain apart from the zinc finger core of the protein. ATP stimulated the nuclease activity in the presence of zinc, and this stimulation required a plant-specific C-terminal domain, again apart from the zinc finger core. These studies reveal a subtle and unexpected complexity to AtCPSF30, and raise the possibility that multiple avenues of regulation may impinge on this protein through different functional domains.

PMID:
18331819
DOI:
10.1016/j.abb.2008.02.027
[Indexed for MEDLINE]

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