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J Fluoresc. 2008 May-Jul;18(3-4):733-9. doi: 10.1007/s10895-008-0334-1. Epub 2008 Mar 11.

Cellular uptake of fluorescent labelled biotin-streptavidin microspheres.

Author information

1
Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, UK.

Abstract

Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin-streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin-biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.

PMID:
18330682
DOI:
10.1007/s10895-008-0334-1
[Indexed for MEDLINE]

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