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Vet J. 2008 Oct;178(1):149-52. doi: 10.1016/j.tvjl.2007.07.023. Epub 2008 Mar 6.

A semi-defined medium without serum for small ruminant mycoplasmas.

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  • 1Unidad de Epidemiología y Medicina Preventiva, Spain. aramirez@dpat.ulpgc.es

Abstract

The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri.

PMID:
18328748
DOI:
10.1016/j.tvjl.2007.07.023
[PubMed - indexed for MEDLINE]
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