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Fertil Steril. 2009 Feb;91(2):602-10. doi: 10.1016/j.fertnstert.2007.11.071. Epub 2008 Mar 6.

Menstrual cycle synchronization, ovarian stimulation, and in vitro fertilization in olive baboons (Papio anubis): a prospective randomized study.

Author information

1
Leuven University Fertility Centre, University Hospital Gasthuisberg, Leuven, Belgium.

Abstract

OBJECTIVE:

To synchronize the baboon menstrual cycle and to compare different methods of ovarian stimulation for IVF in baboons.

DESIGN:

Prospective randomized study.

SETTING:

Institute of Primate Research, Nairobi, Kenya.

ANIMAL(S):

Ten female baboons were treated with an oral contraceptive (33 +/- 8 days) and randomized for ovarian stimulation in two cycles.

INTERVENTION(S):

In cycle 1 (C1), baboons were stimulated as follows: clomiphene citrate (CC) for 5 days followed by antagonist (group CC-1, n = 3); recombinant gonadotropins (rGn: rFSH and rLH) with antagonist (group antagon-1, n = 3); and long GnRH agonist protocol (group LP-1, n = 4). After 1- month rest, in cycle 2 (C2), the baboons were stimulated as follows: CC for 8 days without antagonist (group CC-2, n = 2); short GnRH agonist protocol (group SP-2, n = 4); long GnRH agonist protocol (group LP-2, n = 4). Oocyte aspiration was performed 34-36 hours after injecting 5000 IU recombinant hCG; the oocytes were then fertilized.

MAIN OUTCOME MEASURE(S):

Oocytes retrieved and fertilization rate.

RESULT(S):

Withdrawal bleeding occurred 4 +/- 1 days after the cessation of the contraceptive. Ovarian stimulation using the different protocols resulted in the following mean numbers of retrieved oocytes: LP-1, n = 19; LP-2, n = 19; CC-1, n = 4; CC-2, n = 4; antagon-1, n = 9; and SP-2, n = 14. Fertilization by intracytoplasmic sperm injection varied (23%-54%).

CONCLUSION(S):

The baboon menstrual cycle can be synchronized using an oral contraceptive. A long GnRH agonist protocol for ovarian stimulation may be suitable for ovarian stimulation in baboons.

[Indexed for MEDLINE]

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