Molecular characterisation of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. isolates at a tertiary-care centre in Lebanon

Clin Microbiol Infect. 2008 May;14(5):501-4. doi: 10.1111/j.1469-0691.2008.01964.x. Epub 2008 Mar 4.

Abstract

The prevalence of bla CTX-M, bla TEM and bla SHV genes among extended-spectrum beta-lactamase (ESBL)-producing clinical isolates of Escherichia coli (n = 50) and Klebsiella spp. (n = 50) from Lebanon was 96%, 57% and 67%, and 40%, 82% and 84%, respectively. Genotyping revealed that the clonal diversity was unrelated to the presence of bla genes. Sequence analysis of 16 selected isolates identified the bla CTX-M-15, bla TEM-1, bla OXA-1 and six bla SHV genes, as well as the gene encoding the quinolone-modifying enzyme AAC(6')-Ib-cr. The genes encoding CTX-M-15 and AAC(6')-Ib-cr were carried on a 90-kb plasmid of the pC15-1a or pCTX-15 type, which transferred both ESBL production and quinolone resistance from donors to transconjugants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross Infection / microbiology
  • Enterobacteriaceae Infections / microbiology
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Infections / microbiology
  • Genotype
  • Humans
  • Klebsiella / enzymology
  • Klebsiella / genetics*
  • Lebanon
  • beta-Lactamases / genetics*

Substances

  • beta-Lactamases