Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biol Chem. 2008 Apr 25;283(17):11073-7. doi: 10.1074/jbc.C700242200. Epub 2008 Mar 3.

Direct interaction between SET8 and proliferating cell nuclear antigen couples H4-K20 methylation with DNA replication.

Author information

1
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

Abstract

Chromatin endowed by histone modifications governs chromatin structure, which in turn represents a means to regulate cellular processes, including transcription and heterochromatin formation. Recent evidence revealed a plethora of enzymes that catalyze specific histone modifications for epigenetic maintenance, and dysregulation of which contributes to tumorigenesis and developmental defects. The histone methyltransferase SET8 (also known as Pr-Set7) was previously reported to monomethylate Lys(20) of histone H4. However, the temporal and spatial control of SET8 activity remains elusive. Here, we provide evidence to support that SET8 monomethylates Lys(20) of histone H4 during S phase by tethering to proliferating cell nuclear antigen via a putative proliferating cell nuclear antigen-interacting protein box. In addition, we show that SET8 function is required for S phase progression. Finally, deletion of SET8 in mice causes embryonic lethality, suggesting that SET8 plays an important role in mammalian embryogenesis.

PMID:
18319261
PMCID:
PMC2431066
DOI:
10.1074/jbc.C700242200
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center