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Nano Lett. 2008 Apr;8(4):1041-6. doi: 10.1021/nl072742x. Epub 2008 Mar 5.

Self-contained, biomolecular motor-driven protein sorting and concentrating in an ultrasensitive microfluidic chip.

Author information

1
Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Abstract

We developed a molecular sorter that operates without external power or control by integrating the microtubule-based, biological motor kinesin into a microfluidic channel network to sort, transport, and concentrate molecules. In our devices, functionalized microtubules that capture analyte molecules are steered along kinesin-coated microchannel tracks toward a collector structure, concentrated, and trapped. Using fluorescent analyte molecules and nanoliter sample volumes, we demonstrated 14 fM sensitivity, even in the presence of high concentrations of other proteins.

PMID:
18318504
DOI:
10.1021/nl072742x
[Indexed for MEDLINE]

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