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J Neurosci Methods. 2008 May 15;170(1):149-55. doi: 10.1016/j.jneumeth.2008.01.011. Epub 2008 Jan 20.

Strain fields in histological slices of brain tissue determined by synchrotron radiation-based micro computed tomography.

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1
Computer Vision Laboratory, ETH Zürich, Sternwartstrasse 7, 8092 Zürich, Switzerland.

Abstract

Accurate knowledge of the morphology of the human brain is required for minimally or non-invasive surgical interventions. On the (sub-)cellular level, brain tissue is generally characterized using optical microscopy, which allows extracting morphological features with a wide spectrum of staining procedures. The preparation of the histological slices, however, often leads to artifacts resulting in imperfect morphological data. In addition, the generation of 3D data is time-consuming. Therefore, we propose synchrotron radiation-based micro computed tomography (SRmicroCT) avoiding preparation artifacts and giving rise to the 3D morphology of features such as gray and white matter on the micrometer level. One can differentiate between white and gray matter without any staining procedure because of different X-ray absorption values. At the photon energy of 10keV, the white matter exhibits the absorption of 5.08 cm(-1), whereby the value for the gray matter corresponds to 5.25 cm(-1). The tomography data allow quantifying the local strains in the histological images using registration algorithms. The deformation of histological slices compared to the SRmicroCT in a 2D-2D registration leads to values of up to 6.3%. Mean deformation values for the Nissl-stained slices are determined to about 1%, whereas the myelin-stained slices yield slightly higher values than 2%.

PMID:
18313143
DOI:
10.1016/j.jneumeth.2008.01.011
[Indexed for MEDLINE]
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