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Genome Biol. 2008;9(3):R47. doi: 10.1186/gb-2008-9-3-r47. Epub 2008 Mar 1.

Gene expression-based screening for inhibitors of PDGFR signaling.

Author information

1
Cancer Program, Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge Center, Cambridge, MA 02142, USA. alena.antipova@appliedbiosystems.com

Abstract

Here we describe a proof-of-concept experiment designed to explore the possibility of using gene expression-based high-throughput screening (GE-HTS) to find inhibitors of a signaling cascade, using platelet derived growth factor receptor (PDGFR) signaling as the example. The previously unrecognized ability of aurintricarboxylic acid to inhibit PDGFR signaling, discovered through a screen of 1,739 compounds, demonstrates the feasibility and generalizability of GE-HTS for the discovery of small molecule modulators of any signaling pathway of interest.

PMID:
18312689
PMCID:
PMC2397499
DOI:
10.1186/gb-2008-9-3-r47
[Indexed for MEDLINE]
Free PMC Article

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