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J Mol Biol. 1991 Aug 5;220(3):621-9.

Behavior of a cross-linked attachment site: testing the role of branch migration in site-specific recombination.

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Department of Chemistry, Pennsylvania State University, University Park 16802.


Integrative recombination of bacteriophage lambda requires perfect homology between partners over a short segment of DNA, the overlap region, that separates the positions of top and bottom strand exchange. We constructed a specific cross-link between complementary strands in the overlap region of one partner, using a method designed to introduce minimal distortion of DNA. The modified attachment site could initiate recombination, forming a Holliday junction, but could not resolve this junction so as to complete the recombination. This demonstrates that the ability of complementary base-pairs to dissociate is important for overlap region function and strongly supports the view that branch migration across this region is the way homology is sensed during integrative recombination.

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