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Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3733-8. doi: 10.1073/pnas.0712247105. Epub 2008 Feb 29.

In vitro self-assembly of tailorable nanotubes from a simple protein building block.

Author information

1
Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

We demonstrate a method for generating discretely structured protein nanotubes from the simple ring-shaped building block, homohexameric Hcp1 from Pseudomonas aeruginosa. Our design exploited the observation that the crystal lattice of Hcp1 contains rings stacked in a repeating head-to-tail pattern. High-resolution detail of the ring-ring interface allowed the selection of sites for specific cysteine mutations capable of engaging in disulfide bond formation across rings, thereby generating stable Hcp1 nanotubes. Protein nanotubes containing up to 25 subunits ( approximately 100 nm in length) were self-assembled under simple conditions. Furthermore, we demonstrate that the tube ends and interior can be independently and specifically functionalized to generate nanocapsules.

PMID:
18310321
PMCID:
PMC2268831
DOI:
10.1073/pnas.0712247105
[Indexed for MEDLINE]
Free PMC Article

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