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Chemotherapy. 2008;54(2):101-6. doi: 10.1159/000118661. Epub 2008 Feb 25.

Carbapenem-hydrolyzing oxacillinase, OXA-48, persists in Klebsiella pneumoniae in Istanbul, Turkey.

Author information

1
Department of Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Abstract

BACKGROUND:

Two OXA-48-producing Klebsiella pneumoniae isolates (KP-4936 and KP-154488) were analyzed.

METHOD:

Minimum inhibitory concentrations were determined using agar dilution and E-test, beta-lactamase production by phenotypic tests (E-test MBL and ESBL, isoelectric focusing, and bioassay) and molecular methods (PCR, RAPD-PCR, sequencing, plasmid analysis, and conjugation).

RESULTS:

Isolates were resistant to all beta-lactams, including carbapenems. PCR and sequencing identified bla(OXA-48) in both isolates and the transconjugant. KP-4936 harbored bla(TEM-1) (pI 5.4) and bla(CTX-M-15) genes (pI 8.6), while KP-154488 was positive for bla(TEM-1) (pI 5.4), bla(CTX-M-15) (pI 8.9), and bla(SHV2a) (pI 7.6), in addition. The enzyme with a pI of 7.2 hydrolyzed imipenem according to a bioassay result. Plasmids (70 and 140 kb) from KP-4936 were transferred by conjugation. RAPD-PCR found no clonal relationship between the two strains.

CONCLUSION:

Carbapenem resistance may spread among Enterobacteriaceae via the transferable enzyme OXA-48.

PMID:
18303258
DOI:
10.1159/000118661
[Indexed for MEDLINE]

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