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BMC Dev Biol. 2008 Feb 27;8:21. doi: 10.1186/1471-213X-8-21.

Expression profiling of circulating non-red blood cells in embryonic blood.

Author information

1
Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan. mcintyre@cdb.riken.jp

Abstract

BACKGROUND:

In addition to erythrocytes, embryonic blood contains other differentiated cell lineages and potential progenitor or stem cells homed to changing niches as the embryo develops. Using chicken as a model system, we have isolated an enriched pool of circulating non red blood cells (nRBCs) from E4 and E6 embryos; a transition period when definitive hematopoietic lineages are being specified in the peri-aortic region.

RESULTS:

Transcriptome analysis of both nRBC and RBC enriched populations was performed using chicken Affymetrix gene expression arrays. Comparison of transcript profiles of these two populations, with verification by RT-PCR, reveals in nRBCs an expression signature indicative of hematopoietic stem cells (HSCs) and progenitor cells of myeloid and lymphoid lineages, as well as a number of previously undescribed genes possibly involved in progenitor and stem cell maintenance.

CONCLUSION:

This data indicates that early circulating embryonic blood contains a full array of hematopoietic progenitors and stem cells. Future studies on their heterogeneity and differentiation potentials may provide a useful alternative to ES cells and perinatal blood.

PMID:
18302797
PMCID:
PMC2277405
DOI:
10.1186/1471-213X-8-21
[Indexed for MEDLINE]
Free PMC Article

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