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Cell. 1991 Jul 26;66(2):291-304.

A nexus between Oct-4 and E1A: implications for gene regulation in embryonic stem cells.

Author information

1
Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Göttingen, Federal Republic of Germany.

Abstract

Oct-4 is a transcription factor expressed in the pluripotent progenitor cells of the early mouse embryo. Additional factors are required for the distal activation of genes in differentiated cells containing ectopically expressed Oct-4. Here we show that Oct-4 and E1A are sufficient for distance-independent activation of the basal transcription machinery. The ratio of Oct-4 to E1A is critical for transcriptional activation, because higher levels of either factor are less efficient. Activation depends on a transactivation domain linked to the POU domain of Oct-4 and also on the conserved domain 3 of the 289RE1A protein. This domain is required for binding to the C-terminal part of Oct-4 including the POU domain. Our results indicate that E1A can serve as a bridging factor between Oct-4 and the basal initiation complex, and we postulate that an E1A-like factor acts as a cellular bridging factor of Oct-4 in pluripotent cells.

PMID:
1830243
DOI:
10.1016/0092-8674(91)90619-a
[Indexed for MEDLINE]
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