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PLoS One. 2008 Feb 27;3(2):e1662. doi: 10.1371/journal.pone.0001662.

High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.

Author information

1
Fluidigm Corporation, South San Francisco, California, USA.

Abstract

We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.

PMID:
18301740
PMCID:
PMC2244704
DOI:
10.1371/journal.pone.0001662
[Indexed for MEDLINE]
Free PMC Article

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