Format

Send to

Choose Destination
See comment in PubMed Commons below
Analyst. 2008 Mar;133(3):356-60. doi: 10.1039/b716834a. Epub 2008 Jan 8.

Opto-fluidic micro-ring resonator for sensitive label-free viral detection.

Author information

1
Department of Biological Engineering, University of Missouri-Columbia, 240D, Bond Life Sciences Center, Columbia, MO 65211, USA.

Abstract

We have demonstrated sensitive label-free virus detection using the opto-fluidic ring resonator (OFRR) sensor. The OFRR is a novel sensing platform that integrates the microfluidics and photonic sensing technology with a low detection limit and small volume. In our experiment, filamentous bacteriophage M13 was used as a safe model system. Virus samples were flowed through the OFRR whose surface was coated with M13-specific antibodies. We studied the sensor performance by monitoring in real-time the virus and antibody interaction. It is shown that OFRR can detect M13 with high specificity and sensitivity. The detection limit is approximately 2.3 x 10(3) pfu mL(-1) and the detection dynamic range spanned seven orders of magnitude. Theoretical analysis was also carried out to confirm the experimental results. Our study will lead to development of novel OFRR-based, sensitive, rapid, and low-cost micro total analysis devices for virus detection.

PMID:
18299750
DOI:
10.1039/b716834a
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Royal Society of Chemistry
    Loading ...
    Support Center