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Appl Environ Microbiol. 2008 Apr;74(8):2529-33. doi: 10.1128/AEM.00975-07. Epub 2008 Feb 22.

Species-specific identification of Campylobacters by PCR-restriction fragment length polymorphism and PCR targeting of the gyrase B gene.

Author information

1
National Food Research Institute, Food Hygiene Team, 2-1-12 Kannondai, Tsukuba 305-8642, Japan. skawasa@affrc.go.jp

Abstract

PCR-restriction fragment length polymorphism (RFLP) analysis of a 960-bp fragment of the Campylobacter gyrB gene with either DdeI or XspI restriction enzymes generated unique digestion patterns for 12 different Campylobacter species. In addition, PCR assays using species-specific primer sets targeting gyrB were specific for the respective Campylobacter species. Therefore, PCR-RFLP analysis and species-specific PCR assays based on the gyrB gene provide valuable tools for rapid and unambiguous identification of the majority of Campylobacter species.

PMID:
18296535
PMCID:
PMC2293133
DOI:
10.1128/AEM.00975-07
[Indexed for MEDLINE]
Free PMC Article
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