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Biochem J. 2008 Jun 1;412(2):265-73. doi: 10.1042/BJ20071501.

Cep57, a multidomain protein with unique microtubule and centrosomal localization domains.

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1
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA.

Abstract

The present study demonstrates different functional domains of a recently described centrosomal protein, Cep57 (centrosomal protein 57). Endogenous Cep57 protein and ectopic expression of full-length protein or the N-terminal coiled-coil domain localize to the centrosome internal to gamma-tubulin, suggesting that it is either on both centrioles or on a centromatrix component. The N-terminus can also multimerize with the N-terminus of other Cep57 molecules. The C-terminus contains a second coiled-coil domain that directly binds to MTs (microtubules). This domain both nucleates and bundles MTs in vitro. This activity was also seen in vivo, as overexpression of full-length Cep57 or the C-terminus generates nocodazole-resistant MT cables in cells. Based on the present findings, we propose that Cep57 serves as a link with its N-terminus anchored to the centriole or centromatrix and its C-terminus to MTs.

PMID:
18294141
PMCID:
PMC4351815
DOI:
10.1042/BJ20071501
[Indexed for MEDLINE]
Free PMC Article
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