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Adv Exp Med Biol. 2008;614:65-72. doi: 10.1007/978-0-387-74911-2_8.

Adjuvant induced glucose uptake by activated T cells is not correlated with increased survival.

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1
Institute for Cellular Therapeutics, University of Louisville School of Medicine, 570, S. Preston Street, Louisville, KY 40202, USA.

Abstract

Authors contributed equally to this manuscript Natural adjuvants, such as bacterial lipopolysaccharide (LPS), activate antigen presenting cells via Toll-like receptors and, indirectly, increase the survival of antigen-activated T cells. The molecular mechanisms leading to increased survival remain poorly defined. Because T cell clonal expansion leads to high energy demands, we hypothesized that increased glucose uptake and/or utilization in adjuvant-activated T cells could be important molecular event(s) that would lead to adjuvant-associated T cell survival advantage. Using a fluorescent analog of 2-deoxyglucose, 2-NBDG, we measured glucose accumulation and rate of uptake in T cells from mice treated with antigen in the absence or presence of LPS. Although adjuvant activated T cells increased the accumulation of 2-NBDG, the rate of uptake was unchanged compared to cells activated with only antigen. Furthermore, glucose transport inhibitors, cytochalasin B or phloretin, decreased the accumulation of glucose in adjuvant-treated T cells, but this decrease did not impair adjuvant-associated survival advantages. Together, these data indicate that increased glucose uptake through glucose transporters is not required for increased survival of activated T cells.

PMID:
18290315
DOI:
10.1007/978-0-387-74911-2_8
[Indexed for MEDLINE]
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