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Int Arch Allergy Immunol. 2008;146(3):212-8. doi: 10.1159/000115889. Epub 2008 Feb 11.

A single oral sensitization to peanut without adjuvant leads to anaphylaxis in mice.

Author information

1
EA 3999 Allergic Diseases: Diagnosis and Therapeutics, Department of Internal Medicine, Clinical Immunology and Allergology, University Hospital, Central Hospital, Nancy, France.

Abstract

BACKGROUND:

A model of peanut food allergy has been developed in mice using a simple sensitization protocol leading to a quantitatively measurable allergic response.

METHODS:

C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe anaphylaxis.

RESULTS:

Anaphylactic reaction was evidenced by vascular leakage, severe clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by increased concentrations of serum mouse mast cell protease-1. Sensitization to peanut was demonstrated by positive skin tests (ear swelling test and intradermal skin testing) and increased peanut-specific IgE levels.

CONCLUSIONS:

Thus, we obtained a model of severe peanut hypersensitivity within 2 weeks following single oral exposure without adjuvant. This model may be useful for further basic and applied studies on peanut allergy.

PMID:
18268389
DOI:
10.1159/000115889
[Indexed for MEDLINE]

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