Format

Send to

Choose Destination
Curr Protoc Mol Biol. 2006 Nov;Chapter 7:Unit 7.8. doi: 10.1002/0471142727.mb0708s76.

Polony DNA sequencing.

Author information

1
Harvard Medical School, Boston, Massachusetts, USA.

Abstract

Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.

PMID:
18265387
DOI:
10.1002/0471142727.mb0708s76
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center