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Curr Protoc Mol Biol. 2003 May;Chapter 26:Unit 26.2. doi: 10.1002/0471142727.mb2602s62.

Gene silencing by RNAi in mammalian cells.

Author information

1
Ribopharma AG, Kulmbach, Germany.

Abstract

This unit provides information how to use short interfering RNA (siRNA) for sequence specific gene silencing in mammalian cells. Several ways for siRNA generation and optimisation, as well as recommendations for cell transfection are presented.

PMID:
18265326
DOI:
10.1002/0471142727.mb2602s62
[Indexed for MEDLINE]

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