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Curr Protoc Mol Biol. 2002 Feb;Chapter 17:Unit 17.6. doi: 10.1002/0471142727.mb1706s57.

Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Author information

1
The Johns Hopkins University Medical School, Baltimore, Maryland, USA.

Abstract

The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

PMID:
18265305
DOI:
10.1002/0471142727.mb1706s57
[Indexed for MEDLINE]

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