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Curr Protoc Mol Biol. 2001 May;Chapter 2:Unit2.2. doi: 10.1002/0471142727.mb0202s42.

Preparation of genomic DNA from mammalian tissue.

Author information

1
Harvard Medical School and Beth, Israel Deaconess Medical Center, Boston, Massachusetts, USA.

Abstract

There are a number of different procedures for the preparation of genomic DNA. They all start with some form of cell lysis, followed by deproteination and recovery of DNA. The main differences between various approaches lie in the extent of deproteination and in molecular weight of the DNA produced. The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent SDS.

PMID:
18265182
DOI:
10.1002/0471142727.mb0202s42
[Indexed for MEDLINE]

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