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Curr Protoc Mol Biol. 2001 May;Chapter 11:Unit11.10. doi: 10.1002/0471142727.mb1110s18.

Production of monoclonal antibody supernatant and ascites fluid.

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  • 1University of California School of Medicine, San Francisco, California, USA.


Procedures detailing the production of monoclonal antibody supernatants, including the production of larger amounts (liters) are presented in this unit. An alternate protocol for large-scale production of hybridomas or cells (e.g., for isolation of cellular proteins) involves a similar procedure. A method for producing and obtaining ascites fluid containing the monoclonal antibody is also presented.

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