Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2008 Mar;5(3):235-7. doi: 10.1038/nmeth.1184. Epub 2008 Feb 10.

Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.

Author information

1
Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.

Abstract

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

PMID:
18264105
PMCID:
PMC3439997
DOI:
10.1038/nmeth.1184
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center