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Appl Environ Microbiol. 2008 Apr;74(7):2254-8. doi: 10.1128/AEM.02601-07. Epub 2008 Feb 8.

Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR.

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1
Utah State University, Center for Integrated BioSystems, 4700 Old Main Hill, Logan, UT 84322-4700, USA.

Abstract

We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of approximately 4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

PMID:
18263751
PMCID:
PMC2292596
DOI:
10.1128/AEM.02601-07
[Indexed for MEDLINE]
Free PMC Article
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