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J Surg Res. 2008 Jun 1;147(1):75-8. doi: 10.1016/j.jss.2007.05.042. Epub 2007 Dec 26.

An experimental animal model of postobstructive pulmonary hypertension.

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1
Universitat de Barcelona, Fundació Clínic, Barcelona, Spain.

Abstract

BACKGROUND:

A small experimental animal model of postobstructive pulmonary hypertension (PH) providing insights in the physiopathology of this disease was developed.

MATERIALS AND METHODS:

Male Lewis rats were anesthetized and aleatory manipulated via a left thoracotomy with (group I, n = 10) or without (group II, n = 10) ligation of the left pulmonary artery. Animals were followed for 2 weeks and then sacrificed. Hemodynamic parameters and blood gases were recorded at baseline and 2 weeks after surgical procedure.

RESULTS:

Group I animals developed a significant (P < 0.01) PH (mean pulmonary artery pressure, 32 +/- 6 versus 16 +/- 2 mm Hg; pulmonary vascular resistance, 46 +/- 3 versus 21 +/- 2 mm Hg/mL/min; reduction of cardiac output, 75 +/- 3 versus 105 +/- 4 mL/min), compared to group II animals, and had a significant (P < 0.01) worse gas exchange (partial arterial pressure of O(2): 91 +/- 3 versus 439 +/- 24 mm Hg; partial arterial pressure of CO(2): 54 +/- 3 versus 42 +/- 2 mm Hg, on a fraction of inspired oxygen of 1.0), right ventricle hypertrophy (ventricle to left ventricle/septum ratio, 0.56 +/- 0.04 versus 0.45 +/- 0.04, P < 0.02) and less tolerance test (immobility time, 123 +/- 11 versus 61 +/- 8 s, P < 0.01) than group II animals. Histologically, ligated lungs showed postobstructive pulmonary vasculopathic abnormalities and bronchial-pulmonary artery hypertrophy, and the contralateral lung had initial signs of small vessel arteriopathy.

CONCLUSIONS:

The experimental model generated here successfully reproduced a PH morphologically and functionally similar to clinical postembolic PH and might be used for evaluating the physiopathology of postobstructive PH.

PMID:
18262548
DOI:
10.1016/j.jss.2007.05.042
[Indexed for MEDLINE]
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