Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes

Holy N Rabetafika; Cédric Gigot; Marie-Laure Fauconnier; Marc Ongena; Jacqueline Destain; Patrick du Jardin; Jean-Paul Wathelet; Philippe Thonart

doi:10.1007/s10529-008-9652-2

Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes

Biotechnol Lett. 2008 Jun;30(6):1115-9. doi: 10.1007/s10529-008-9652-2. Epub 2008 Feb 8.

Authors

Holy N Rabetafika¹, Cédric Gigot, Marie-Laure Fauconnier, Marc Ongena, Jacqueline Destain, Patrick du Jardin, Jean-Paul Wathelet, Philippe Thonart

Affiliation

¹ General and Organic Chemistry Unit, Gembloux Agricultural University, 5030, Gembloux, Belgium. rabetafika.h@fsagx.ac.be

PMID: 18259877
DOI: 10.1007/s10529-008-9652-2

Abstract

Hydroperoxide lyase activity was found in sugar beet leaves. Its optimum pH and temperature were, respectively, 6.7 and 22 degrees C. Under these conditions, conversion of linolenic acid 13-hydroperoxide to cis-3-hexenal with a maximum yield of 80% was reached after only 2 min. The stability of cis-3-hexenal was improved by acidifying the reaction medium. Based on these studies, a bioprocess producing green-note aldehydes in a laboratory-scale was achieved.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldehyde-Lyases / isolation & purification
Aldehyde-Lyases / metabolism*
Aldehydes / metabolism*
Beta vulgaris / enzymology*
Chromatography, High Pressure Liquid
Cytochrome P-450 Enzyme System / isolation & purification
Cytochrome P-450 Enzyme System / metabolism*
Hexobarbital / metabolism
Hydrogen-Ion Concentration
Linoleic Acids / metabolism
Linolenic Acids / metabolism
Lipid Peroxides / metabolism
Plant Leaves / enzymology

Substances

Aldehydes
Linoleic Acids
Linolenic Acids
Lipid Peroxides
13-hydroperoxylinolenic acid
Cytochrome P-450 Enzyme System
Hexobarbital
Aldehyde-Lyases
hydroperoxide lyase
13-hydroperoxylinoleic acid