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Biotechniques. 2008 Jan;44(1):91-5.

A microfluidic system for dynamic yeast cell imaging.

Author information

1
CellASIC Corporation, San Leandro, CA 94577, USA. pjlee@cellasic.com

Abstract

The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.

PMID:
18254385
DOI:
10.2144/000112673
[Indexed for MEDLINE]
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