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Nature. 1991 Feb 7;349(6309):494-9.

PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome.

Author information

1
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.

Abstract

The assembly of the spliceosome is an ATP-dependent process. The splicing factor PRP16 contains variations of several motifs that define the eIF-4A-like ATP-dependent RNA helicase family. The protein has now been purified and shown to exhibit RNA-dependent ATPase activity. PRP16 is required specifically for the second catalytic step of the splicing reaction in vitro. This function requires ATP binding and/or hydrolysis, which appears to be concomitant with release of the protein from the spliceosome. PRP16 may be the prototype for a set of splicing factors which use ATP to drive a cycle of conformational changes.

PMID:
1825134
DOI:
10.1038/349494a0
[Indexed for MEDLINE]

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