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Biotechnol Lett. 2008 Jun;30(6):1037-40. doi: 10.1007/s10529-008-9638-0. Epub 2008 Feb 5.

Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media.

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1
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan. noritcm@mbox.nc.kyushu-u.ac.jp

Abstract

The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40 degrees C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.

PMID:
18250967
DOI:
10.1007/s10529-008-9638-0
[Indexed for MEDLINE]
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