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Anal Bioanal Chem. 2008 Mar;390(6):1585-94. doi: 10.1007/s00216-008-1866-6. Epub 2008 Feb 3.

Quantitative micro-analysis of metal ions in subcellular compartments of cultured dopaminergic cells by combination of three ion beam techniques.

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1
Groupe d'Imagerie Chimique Cellulaire et Spéciation, Chemin du solarium, Laboratoire de Chimie Nucléaire Analytique et Bioenvironnementale, Universités de Bordeaux 1 & 2, 33175, Gradignan, France.

Abstract

Quantification of the trace element content of subcellular compartments is a challenging task because of the lack of analytical quantitative techniques with adequate spatial resolution and sensitivity. Ion beam micro-analysis, using MeV protons or alpha particles, offers a unique combination of analytical methods that can be used with micrometric resolution for the determination of chemical element distributions. This work illustrates how the association of three ion beam analytical methods, PIXE (particle induced X-ray emission), BS (backscattering spectrometry), and STIM (scanning transmission ion spectrometry), allows quantitative determination of the trace element content of single cells. PIXE is used for trace element detection while BS enables beam-current normalization, and STIM local mass determination. These methods were applied to freeze-dried cells, following a specific cryogenic protocol for sample preparation which preserves biological structures and chemical distributions in the cells. We investigated how iron accumulates into dopaminergic cells cultured in vitro. We found that the iron content increases in dopaminergic cells exposed to an excess iron, with marked accumulation within distal ends, suggesting interaction between iron and dopamine within neurotransmitter vesicles. Increased iron content of dopaminergic neurons is suspected to promote neurodegeneration in Parkinson's disease.

PMID:
18246461
DOI:
10.1007/s00216-008-1866-6
[Indexed for MEDLINE]
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