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Antiviral Res. 2008 Mar;77(3):232-6. doi: 10.1016/j.antiviral.2007.12.009. Epub 2008 Jan 16.

Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay.

Author information

1
Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.

Abstract

A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32 microM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity.

PMID:
18243348
DOI:
10.1016/j.antiviral.2007.12.009
[Indexed for MEDLINE]

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