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J Microbiol Methods. 2008 Mar;72(3):321-5. doi: 10.1016/j.mimet.2007.12.015. Epub 2008 Jan 12.

A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells.

Author information

1
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA.

Abstract

Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication.

PMID:
18242746
PMCID:
PMC2276870
DOI:
10.1016/j.mimet.2007.12.015
[Indexed for MEDLINE]
Free PMC Article

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