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FEBS Lett. 2008 Mar 5;582(5):643-50. doi: 10.1016/j.febslet.2008.01.035. Epub 2008 Jan 31.

R-spondin1 synergizes with Wnt3A in inducing osteoblast differentiation and osteoprotegerin expression.

Author information

1
Department of Biochemistry and Molecular Biology, Drug Discovery Division, Southern Research Institute, Birmingham, AL 35255, USA.

Abstract

R-spondins are a new group of Wnt/beta-catenin signaling agonists, however, the role of these proteins in bone remains unclear. We reported herein that R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/beta-catenin signaling in the uncommitted mesenchymal C2C12 cells. Furthermore, we found that Rspo1 at concentrations as low as 10 ng/ml synergized strongly with Wnt3A to induce C2C12 osteoblastic differentiation and osteoprotegerin expression. These events were blocked by Wnt/beta-catenin signaling antagonist Dickkopf-1. Finally, we demonstrated that Rspo1 synergized with Wnt3A to induce primary mouse osteoblast differentiation. Together, these findings suggest that Rpos1 may play an important role in bone remodeling.

PMID:
18242177
DOI:
10.1016/j.febslet.2008.01.035
[Indexed for MEDLINE]
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