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J Microbiol Methods. 2008 Jun;73(3):269-72. doi: 10.1016/j.mimet.2007.11.014. Epub 2007 Nov 22.

A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis.

Author information

1
Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011-5640, United States.

Abstract

The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

PMID:
18237793
DOI:
10.1016/j.mimet.2007.11.014
[Indexed for MEDLINE]

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