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Methods Enzymol. 2008;436:21-33. doi: 10.1016/S0076-6879(08)36002-9.

Delivery of nitric oxide for analysis of the function of cytochrome c'.

Author information

1
Department of Biology, University of York, Heslington, York, United Kingdom.

Abstract

On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.

PMID:
18237625
DOI:
10.1016/S0076-6879(08)36002-9
[Indexed for MEDLINE]

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