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Biotechnol J. 2008 Mar;3(3):311-24. doi: 10.1002/biot.200700222.

The BRET technology and its application to screening assays.

Author information

1
UMR 8090, CNRS, Université Lille 2, Lille, France.

Abstract

The bioluminescence resonance energy transfer (BRET) method is based on resonance energy transfer between a light-emitting enzyme and a fluorescent acceptor. Since its first description in 1999, several versions of BRET have been described using different substrates and energy donor/acceptor couples. Today, BRET is considered as one of the most versatile techniques for studying the dynamics of protein-protein interactions in living cells. Various studies have applied BRET-based assays to screen new receptor ligands and inhibitors of disease-related-proteases. Inhibitors of protein-protein interactions are likely to become a new major class of therapeutic drugs, and BRET technology is expected to play an important role in the identification of such compounds. This review describes the original BRET-based methodology, more recent variants, and potential applications to drug screening.

PMID:
18228541
DOI:
10.1002/biot.200700222
[Indexed for MEDLINE]

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