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Curr Protoc Cell Biol. 2001 May;Chapter 4:Unit 4.3. doi: 10.1002/0471143030.cb0403s00.

Immunofluorescence staining.

Author information

1
National Heart, Lung, and Blood Institute/NIH, Bethesda, Maryland, USA.

Abstract

This unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. This provides both a visible signal and amplification of the signal and the results are observed with a fluorescent microscope.

PMID:
18228363
PMCID:
PMC4709840
DOI:
10.1002/0471143030.cb0403s00
[Indexed for MEDLINE]
Free PMC Article

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