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Dig Dis Sci. 2008 Sep;53(9):2550-5. doi: 10.1007/s10620-007-0153-2. Epub 2008 Jan 26.

The activity of class I, II, III, and IV alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in liver cancer.

Author information

1
Department of Biochemical Diagnostics, Medical Academy, Skłodowskiej-Curie 24 A, Bialystok, Poland. wjelski@amb.edu.pl

Abstract

BACKGROUND:

Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are most abundant in the liver, are the main enzymes involved in ethanol and acetaldehyde metabolism.

AIMS:

The purpose of this study was to compare the activity of ADH isoenzymes and ALDH between liver carcinoma cells and healthy hepatocytes.

PATIENTS:

Samples were taken from 44 liver cancer patients (19 drinkers, 25 nondrinkers). Seventeen patients had primary liver tumors and 27 had metastatic liver tumors.

METHODS:

Fluorimetric methods were used for measurement of the activity of class I and II ADH isoenzymes and ALDH activity. Total ADH activity and activity of class III and IV isoenzymes were measured by a photometric method.

RESULTS:

The activities of total ADH, ALDH, and class I ADH were significantly higher in cancer cells than in healthy tissues. The other tested classes of ADH showed a tendency toward higher activity in cancer than in normal cells. The differences between enzymes of drinkers and nondrinkers in both cancer and healthy tissue, were not significant.

CONCLUSION:

Differences in the activities of total ADH, ALDH and class I ADH isoenzyme between cancer liver tissues and healthy hepatocytes might be a factor in ethanol metabolism disorders, which can intensify carcinogenesis.

PMID:
18224440
DOI:
10.1007/s10620-007-0153-2
[Indexed for MEDLINE]

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