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Nano Lett. 2008 Feb;8(2):619-23. doi: 10.1021/nl073028i. Epub 2008 Jan 26.

Gold nanostoves for microsecond DNA melting analysis.

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Photonics and Optoelectronics Group, Physics Department, CeNS, Ludwig-Maximilians-Universität München, Amalienstrasse 54, 80799 Munich, Germany.


In traditional DNA melting assays, the temperature of the DNA-containing solution is slowly ramped up. In contrast, we use 300 ns laser pulses to rapidly heat DNA bound gold nanoparticle aggregates. We show that double-stranded DNA melts on a microsecond time scale that leads to a disintegration of the gold nanoparticle aggregates on a millisecond time scale. A perfectly matching and a point-mutated DNA sequence can be clearly distinguished in less than one millisecond even in a 1:1 mixture of both targets.

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