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Brief Funct Genomic Proteomic. 2007 Dec;6(4):302-12. doi: 10.1093/bfgp/elm035. Epub 2008 Jan 24.

Improving yeast two-hybrid screening systems.

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Translational Research, and Genomics and Proteomics Core Facilities, German Cancer Research Center, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.


Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein-protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein-protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approaches are needed for proteome-wide interaction screens. In this review, we discuss and compare different approaches for adaptation of Y2H screening to high-throughput, the limits of the method and possible alternative approaches to complement the mapping of organism-wide protein-protein interactions.

[Indexed for MEDLINE]

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