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Methods Mol Biol. 2007;383:215-25.

Yeast and mammalian two-hybrid systems for studying protein-protein interactions.

Author information

1
Burnham Institute for Medical Research, La Jolla, CA, USA.

Abstract

An important step in the analysis of protein function is identification of the interaction partners of each protein. The two-hybrid system has been widely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using either yeast or mammalian cells will be described in this chapter.

PMID:
18217688
DOI:
10.1007/978-1-59745-335-6_14
[Indexed for MEDLINE]

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