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Zoolog Sci. 2007 Aug;24(8):781-6.

Detection of gamma-tubulin in spermatogonial cells of Bombyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera).

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  • 1Laboratory of Developmental Biology, Rakuno Gakuen University, Ebetsu 069-8501, Japan.


We detected a putative gamma-tubulin gene in silico and detected BACs containing the gene from a Bombyx mori BAC library. BAC-FISH mapping revealed that the gene is located on chromosome 5. To observe the distribution of gamma-tubulin, we employed antibodies against mammalian gamma-tubulin peptides. Western blot analysis disclosed a band very similar in size to gamma-tubulin protein in other species (approximately 48 kDa). In mitotic metaphase of B. mori spermatogonial cells, gamma-tubulin is exclusively localized in the spindle poles, where the centrosomes occur. We applied the same system to the grasshopper Chortophaga viridifasciata, as a representative of insect orders in which the gamma-tubulin distribution had not previously been studied. Gamma-tubulin was also found in the spindle poles during metaphase of spermatogonial cells in the grasshopper.

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