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Anal Chim Acta. 2008 Feb 11;608(2):204-10. doi: 10.1016/j.aca.2007.12.026. Epub 2008 Jan 3.

Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection.

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Department of Analytical Chemistry, Faculty of Chemistry, 41012 Seville, Spain.


A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively. A linear response was observed at plasma levels ranged between 0.3 and 30 microgmL(-1) for imipenem and 1.5 and 20 microgmL(-1) for rifampicin. The detection limits were 0.07 microgmL(-1) and 0.47 microgmL(-1) for imipenem and rifampicin, respectively. The method was applied to the determination of both compounds in mouse plasma samples.

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