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Nat Methods. 2008 Feb;5(2):175-7. doi: 10.1038/nmeth.1178. Epub 2008 Jan 20.

Printing protein arrays from DNA arrays.

Author information

1
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK.

Abstract

We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.

PMID:
18204456
DOI:
10.1038/nmeth.1178
[Indexed for MEDLINE]

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