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FEBS J. 2008 Feb;275(4):671-81. doi: 10.1111/j.1742-4658.2007.06228.x. Epub 2008 Jan 12.

Interactions of elongation factor EF-P with the Escherichia coli ribosome.

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  • 1University of Toronto, C.H. Best Institute, Canada.


EF-P (eubacterial elongation factor P) is a highly conserved protein essential for protein synthesis. We report that EF-P protects 16S rRNA near the G526 streptomycin and the S12 and mRNA binding sites (30S T-site). EF-P also protects domain V of the 23S rRNA proximal to the A-site (50S T-site) and more strongly the A-site of 70S ribosomes. We suggest that EF-P: (a) may play a role in translational fidelity and (b) prevents entry of fMet-tRNA into the A-site enabling it to bind to the 50S P-site. We also report that EF-P promotes a ribosome-dependent accommodation of fMet-tRNA into the 70S P-site.

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