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Blood. 2008 Apr 1;111(7):3880-3. doi: 10.1182/blood-2007-08-107144. Epub 2008 Jan 16.

The TNF (-308A) polymorphism is associated with microchimerism in transfused trauma patients.

Author information

1
Department of Laboratory Medicine, University of California, San Francisco, USA.

Abstract

Microchimerism (MC), defined as the persistence of allogeneic cells at low concentrations, is well documented in transfused trauma patients. We hypothesized that genetic polymorphisms linked to cytokine production could contribute to trauma-induced immune modulation and development of microchimerism after transfusion of trauma patients. We used high-throughput SYBR-green-based genotyping of single nucleotide polymorphisms (SNPs) to characterize 59 transfused trauma patients, with MC (n=30) and without MC (n=29), for 4 functionally significant SNPs: TNF (-308), IL 10 (-1082), IFNG (+874), and TGFB1 (+915). We then compared likelihood for development of MC and the magnitude of immune suppression among subjects with and without these selected immune response SNPs. We identified a significant association between TNF (-308A) SNP and both development of MC and diminished immune responsiveness. Hence predisposing genetic factors may explain, in part, why only a subset of trauma patients develops transfusion-associated microchimerism.

PMID:
18199828
PMCID:
PMC2275039
DOI:
10.1182/blood-2007-08-107144
[Indexed for MEDLINE]
Free PMC Article

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